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. 2014 Jul 22;166(1):217–234. doi: 10.1104/pp.114.243626

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Figure 6. — A typical example of Medicago spp. NBS gene evolution showing three different duplication types: local tandem duplication, ectopic duplication, and segmental duplication. A, Collinearity shared by Medicago spp. NBS gene loci 68 and 69 on chromosome 5 and locus 134 on chromosome 8. NBS genes are indicated by red boxes, non-NBS genes are indicated by white boxes, with syntenic genes linked by lines. B, Phylogenetic analysis revealed three monophyletic NBS gene groups (A, B, and B′). Two relevant Arabidopsis sequences were used as an outgroup, and all major nodes were supported with high confidence. C, A postulated evolutionary history of these NBS genes: steps 1 and 2 occurred before the Medicago spp. lineage separated from the other legumes, and step 3 occurred mainly in the Medicago spp. lineage.