Figure 8.

An evolutionary history of the legume nTNL family containing known functional genes Rpg1-b and Rsv1. A, The reconstructed phylogeny of NBS genes belonging to nTNL-legume family 45 with the soybean Rsv1 gene incorporated. A total of 15 subfamilies that had evolved in the common ancestor of pigeon pea, common bean, and soybean are labeled. Soybean Rpg1-b and Rsv1 are indicated by red triangles. B, NBS gene subfamilies were differentially lost among later-evolving legume lineages. Common ancestors (α, β, and γ) are indicated by pink dots. The presence/absence of 15 subfamilies in pigeon pea, common bean, soybean, and common ancestor β and γ are indicated with purple/gray bars. C, Graph showing the recombination event that occurred on soybean Rpg1-b. Major parental sequence (PI96983 Rsv1) and minor parental sequence (13g26310) are shown in light blue and purple, respectively. Also, the sequence recombination boundary before the NBS domain is shown with a 111-bp-long alignment.