Figure 4.

Levels of IGs in seedlings of double myb bhlh mutants. Double loss-of-function mutant combinations of MYB51 and MYB34 with bHLH04 (A), MYB51 and MYB34 with bHLH05 (B), and MYB51 and MYB34 with bHLH06 (C). For GSL analysis, 3-week-old seedlings were harvested and the sum of the three major IGs (I3M, 1MO-I3M, and 4MO-I3M) is shown. Results are means ± se from three independent experiments with four biological replicates each (n = 12). Values marked with letters are significantly different from other letters (Student’s t test; P < 0.05). DW, Dry weight.