Table II. Levels of primary metabolites in dry seeds.
Values are means ± sd (peak area per 1,000) and ratios as indicated. sd values represent five replicates. Asterisks indicate Student’s t test significance at *P < 0.05, **P < 0.01, and ****P < 5 × 10−4.
| Group | Compound | Wild Type | apx6-1 | Ratio, apx6-1:Wild Type |
|---|---|---|---|---|
| Sugars | Fru monophosphate | 2,725 ± 871 | 3,633 ± 1,679 | 1.3 |
| Gluconic acid | 872 ± 168 | 1,362 ± 489* | 1.6 | |
| Glc biphosphate | 3,629 | 5,050 ± 1,155 | 1.4 | |
| Glc monophosphate | 25,475 ± 9,644 | 34,960 ± 6,984 | 1.4 | |
| Suc | 111,163 ± 13,640 | 144,169 ± 33,243 | 1.3 | |
| Polyols | Erythritol | 494 ± 129 | 634 ± 82* | 1.3 |
| Glycerol | 4,114 ± 402 | 4,847 ± 622 | 1.2 | |
| Myoinositol | 11,692 ± 691 | 11,003 ± 2,911 | 0.9 | |
| Sorbitol | 1,789 ± 748 | 2,259 ± 1,386 | 1.3 | |
| Xylitol | 161 ± 16 | 150 ± 39 | 0.9 | |
| Organic acids | Citrate | 4,727 ± 839 | 10,510 ± 2,214**** | 2.2 |
| Fumarate | 1,568 ± 322 | 8,963 ± 2,432**** | 5.7 | |
| Malate | 2,294 ± 321 | 6,071 ± 1,625**** | 2.6 | |
| Succinate | 337 ± 54 | 471 ± 83** | 1.4 | |
| Benzoate | 3,245 ± 346 | 4,801 ± 489**** | 1.5 | |
| Nicotinic acid | 1,687 ± 148 | 3,177 ± 327**** | 1.9 | |
| Palmitate | 2,765 ± 1,005 | 3,895 ± 2,220 | 1.4 | |
| Pyro-Glu | 31,141 ± 8,245 | 55,645 ± 16,678** | 1.8 | |
| Miscellaneous organic acid | Ethanolamine | 10,923 ± 4,439 | 9,078 ± 2,216 | 0.8 |
| Inorganic acids | Phosphoric acid | 1,066 ± 203 | 2203 ± 830** | 2.1 |
| Hydroxylamine | 5,345 ± 528 | 7,277 ± 1,739* | 1.4 |