Anion contents in the candidate genes. A to C, Nitrate (A), phosphate (B), and sulfate (C) contents were determined in leaves of Arabidopsis T-DNA lines for genes potentially affecting anion homeostasis by HPLC. Lines originating from a nitrate screen are presented in black, those from the phosphate data set are in light gray, and lines from the sulfate screen are shown in dark gray. D, Anion levels in the clcA mutant and its corresponding wild type, Ws. Asterisks mark values significantly different from the wild type at P < 0.05 (Student’s t test). FW, Fresh weight.