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. 2014 Aug 18;14:77. doi: 10.1186/1472-6750-14-77

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Copyright © 2014 Stachowiak et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Activity of purified LLO on Jurkat cell line. LLO concentration dependence, temperature dependence and conditions of storage were compared. Presented values show the percentage of living cells after exposure to LLO. Cytotoxicity of LLO at 22°C and 37°C were compared on preparations without protease inhibitors, and the influence of storage for 25 days (at −70°C) of LLO preparations on cytotoxicity in the presence of protease inhibitors was assessed. FP –freshly prepared LLO preparation; ST- stored LLO preparation. Median values; P₂₅ and P₇₅, n = 3, p < 0.0001 between FP and ST. Differences in cytotoxic activity at 22°C and 37°C were not significant.