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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Bone. 2014 Jul 9;67:81–94. doi: 10.1016/j.bone.2014.06.040

Figure 9. TNAP deficient calvarial cell matrix deposition, adhesion, proliferation and apoptosis.

Figure 9

(A,B) Cells were cultured with ascorbate to induce osteoblast differentiation and collagen was stained with Sirius Red. Staining was quantified by densitometry. Results are shown as means +/− standard deviations of triplicate experiments. Note lack of cell alignment in in TNAP shRNA expressing, as compared to control cells. (C,D) Photograph and quantification of cell adhesion assay. Cell adhesion (E) and mineralization (F) assays were performed on collagen pre-coated plates and quantified. (G) Cells were stained with trypan blue and counted at indicated time points after plating to assay for proliferation. (H) Cells were cultured with 10% FBS (fetal bovine serum) or 0.5% FBS to induce apoptosis. Generation of apoptotic changes in DNA was assayed by a colorimetric reaction. Results are shown as means +/− standard deviations of triplicate experiments.