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. Author manuscript; available in PMC: 2015 May 22.
Published in final edited form as: J Vasc Res. 2014 May 22;51(3):175–189. doi: 10.1159/000360765

Figure 2.

Figure 2

Lower and higher magnifications of VSM cells from cerebral arteries from normal rats showing clustering of mitochondria (m) and sarcoplasmic reticulum (SR) in distinct locations. Arteries were fixed in 2.5% glutaraldehyde and post-fixed in 1% osmium tetroxide and embedded in Epon812. Ultrathin sections (80-90 nm) were mounted on formvar-coated nickel grids (200 mesh), air dried, and stained with 4.7% uranyl acetate and lead citrate (at 10 min and 2 min, respectively). The sections were put on grids and viewed using a FEI Tecnai BioTwin 120 keV TEM with a digital imaging setup (Wake Forest University Health Sciences, Winston-Salem, NC).