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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Gene. 2014 Jun 2;550(1):1–9. doi: 10.1016/j.gene.2014.05.044

Fig. 1.

Fig. 1

The runx2-P1 isoform is upregulated during MC3T3-E1 differentiation. (A) The Runx2 gene produces two predominant message isoforms transcribed from the P1 and P2 promoters. The Runx2-P1 promoter is defined by a previously discovered hypersensitive 0.4-kb “core” sequence (−480 to −1). The consensus ATG begins at +408 of Exon-1 (black region). (B) MC3T3 cultures show elevated bone-related transcript levels throughout the first 9 days post-switching to differentiation conditions as determined by qPCR. Relative levels at d0, d2, d4, d6, and d9 cultures are plotted bone-sialoprotein (ibsp, blue), osteocalcin (bglap2, red), runx2-P1 isoform (green), runx2-P2 isoform (purple). Error bars represent 1STD. (C) Osteoblast cultures display increasing alkaline phosphatase activity (ALP), a hallmark of cultures undergoing matrix-deposition. Select phase images (left column) of cultures throughout d0 to d9, accompanied by ALP stained parallel cultures (right column).