(A) Schematic of anatomical regions of corpus callosum (CC), cerebral cortex (CTX), and ventricle (V) presented in (B) and experimental timeline for chronic hypoxic rearing.
(B) Images showing hypomyelination, OL-lineage HIF1α expression, and OPC maturation arrest in CC of hypoxic WT mice or normoxic Sox10-cre, VHL(fl/fl) mice at P11. Arrowheads denote double-positive cells. Scale bar: 100µm (MBP), 50µm (Olig2).
(C) Immunopurified OPCs exposed to hypoxia or isolated from Plp-creERT2, VHL(fl/fl) mice show differentiation block. Scale bar: 100µm.
(For quantifications, mean+SEM; n≥3 experiments/genotype; **p<0.01, ***p<0.001; one-way ANOVA with Dunnett’s multiple comparison test)
See also Figure S1.