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. Author manuscript; available in PMC: 2015 Jul 17.
Published in final edited form as: Cell. 2014 Jul 10;158(2):383–396. doi: 10.1016/j.cell.2014.04.052

Figure 1. Oligodendrocyte-specific VHL deletion inhibits differentiation and myelination.

Figure 1

(A) Schematic of anatomical regions of corpus callosum (CC), cerebral cortex (CTX), and ventricle (V) presented in (B) and experimental timeline for chronic hypoxic rearing.

(B) Images showing hypomyelination, OL-lineage HIF1α expression, and OPC maturation arrest in CC of hypoxic WT mice or normoxic Sox10-cre, VHL(fl/fl) mice at P11. Arrowheads denote double-positive cells. Scale bar: 100µm (MBP), 50µm (Olig2).

(C) Immunopurified OPCs exposed to hypoxia or isolated from Plp-creERT2, VHL(fl/fl) mice show differentiation block. Scale bar: 100µm.

(For quantifications, mean+SEM; n≥3 experiments/genotype; **p<0.01, ***p<0.001; one-way ANOVA with Dunnett’s multiple comparison test)

See also Figure S1.