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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Allergy Clin Immunol. 2014 Sep;134(3):671–678.e4. doi: 10.1016/j.jaci.2014.06.024

Figure 2. Lineage-negative (Lin−) cells are necessary for IL-25- and IL-33-induced IL-13 production.

Figure 2

PBMCs from subjects with who had allergic asthma and were skin test-positive for house dust mite (HDM) were separated into two fractions, the Lin+ with Lin− cell fraction and the Lin+ cell alone fraction. The Lin+ with Lin− cell fraction contained 82.0% Lin+ cells and 18.0% Lin− cells; the Lin+ cell alone fraction contained 99.5% Lin+ cells and 0.5% Lin− cells. The fractions were cultured with HDM extract (25 μg/ml), IL-25 or IL-33 (10 ng/ml) plus IL-2 (20 U/ml), or combinations of HDM extract and cytokines for 7 days. (A) IL-13 levels in cell-free supernatants of the Lin+ with Lin− cell fraction were determined. (B) IL-13 levels in cell-free supernatants of the Lin+ cell alone fraction are presented as the ratio of the Lin+ cell alone fraction divided by the Lin+ with Lin− cell fraction from the same donor. Data are the mean ± SEM of 3 subjects.