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. Author manuscript; available in PMC: 2014 Aug 31.
Published in final edited form as: Cytometry A. 2012 Dec 13;83(1):150–160. doi: 10.1002/cyto.a.22240

Figure 1.

Figure 1

Regions and gates used in mutivariate analysis. This example shows a lung adenocarcinoma. Logical gates applied to each histogram are shown above the histogram frame. The “Clean Non Heme” gate was created on CD45-/CD14-/CD33-/glycophorin A-singlet events with DNA content ≥ 2N (not shown). The region numbers indicate the markers used for multivariate analysis and are keyed to Table 1. CKP + cytokeratin+, CKN = cytokeratin negative Euploid = gated on region 4 or 9. Aneuploid = gated on region 5 or 10. The euploid and low light scatter regions were matched to tissue infiltrating lymphocytes (not shown).