Table 1.
Possible Validations for Candidate Targets of EBV’s miRNAs
| MRNA | miRNA | mRNA Functions | PAR-CLIP | Ref. | |
|---|---|---|---|---|---|
| Gottwein et al. | Skalsky et al. | ||||
| Bim (BCL2L11) | Various (BART9, 11+12) | Pro-apoptotic; inhibits Bcl-2 | − | ? | [26] |
| BRUCE (birc6) | BART 15-3p | Inhibitor of apoptosis (IAP) | − | − | [29] |
| C1orf109 | BART 2-5p# | Cancer cell proliferation (proposed) | − | + | [38] |
| CAPRIN2 | BART 13-3p | Wnt signaling | − | ? | [27] |
| CASP3 | BART 1-3p | Pro-apoptotic; death protease | + | − | [24]† |
| CLEC2D(LLT1) | BART 1-3p, 3-3p | Ligand for NK receptor | − | + | [28] |
| CXCL11 | BHRF1-3 | IFN-inducible chemokine | ? | − | [32] |
| DAZAP2 | BART 3 | Wnt signaling | + | + | [28] |
| DICE1 | BART 3* | DEAD box protein; tumor suppressor | − | − | [39] |
| DICER1 | BART 6-5p | RNAi processing (miRNA maturation) | − | ? | [40] |
| IPO7 | BART 3 | Nuclear import | + | + | [24], [38]† |
| LY75 | BART 1-5p | Mediates antigen presentation | − | + | [28] |
| MICB | BART 2-5p | Activates NK cell response | − | − | [34] |
| NLRP3 | BART 11-5p, 15 | Pro-apoptotic; release of IL-1B | − | ? | [35] |
| PDCD1LG2 | BHRF1-2-5p, BART 1-5p, 15-3p# | Ligand for PD-1; Immune regulation | ? | + | [38] |
| PDE7A | BART 3 | Cytokine production | − | + | [38] |
| PELI1 | BART 3 | E3 ubiquitin ligase; activation of NF-kB | − | + | [28] |
| PUMA (BBC3) | BART 5 | Pro-apoptotic; inhibits Bcl-2 | − | ? | [25] |
| SP100 | BART 1-5p | Anti-viral response; part of PML-NB | + | + | [28] |
| T-bet (TBX21) | BART 20-5p | Transcription factor, T cell development | − | ? | [41] |
| TOMM22 | BART 16 | Mitochondrial import | + | ? | [38] |
| ZNF451 | BHRF1-2 | Transcriptional regulation? | ? | + | [28] |
+ Clusters of sequence changes in RNAs in RISC consistent with binding of a given EBV miRNA with high confidence (for details see Gottwein et al. [37] and Skalsky et al. [28])
− No clusters of sequence changes in RNAs in RISC consistent with binding of a given EBV miRNA with high confidence (for details see Gottwein et al. [37] and Skalsky et al. [28])
†
Reference proposes additional EBV miRNAs target given mRNA that are not confirmed by PAR-CLIP.
? Some EBV miRNAs are not expressed in the cells analyzed by PAR-CLIP making the PAR-CLIP uninformative for this mRNA.