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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Curr Opin Virol. 2014 Jul 1;0:79–87. doi: 10.1016/j.coviro.2014.05.005

Figure 2.

Figure 2

Shotgun Glycomics is a functional approach to glycomic analysis of cells and tissues. We used it to explore the repertoire of glycans in pig lung that may function as productive influenza virus receptors. The N- and O-linked glycans and glycolipid-derived glycans are released from tissue by chemical or enzymatic methods (1), labeled with a fluorescent tag (2) and separated into individual glycan components by multidimensional chromatography (3) to produce a TGL (4). The components of the TGL are then printed as a microarray and interrogated to identify relevant glycans (5). Glycans of interest are then retrieved from the TGL and subjected to structural analysis using Metadata Assisted Glycan Sequencing (MAGS) techniques (6). As the glycans in the TGL are structurally defined, the Shotgun Glycan Array becomes a Defined Glycan Array and a resource for determining specificities of other viruses and glycan binding proteins.