Table 3.
Sex-specific differences in soleus muscle protein abundance.
| Description | Protein ID | Ref# | F-LCR | M-LCR | F-HCR | M-HCR | Fold diff. |
|---|---|---|---|---|---|---|---|
| Spots significantly more abundant in males | |||||||
| 60 kDa heat shock protein, mitochondrial | CH60 | 381 | 1.414 ± 0.094 | 0.746 ± 0.116 | 1.440 ± 0.271 | 0.755 ± 0.120 | 1.71 |
| Glutathione S-transferase | GSTA3 | 772 | 1.174 ± 0.156 | 0.918 ± 0.209 | 1.262 ± 0.243 | 0.871 ± 0.192 | 1.44 |
| aDesmin | DESM | 351 | 1.070 ± 0.169 | 0.896 ± 0.095 | 1.174 ± 0.133 | 0.963 ± 0.107 | 1.28 |
| Carbonic anhydrase 3 | CAH3 | 741 | 0.959 ± 0.089 | 0.803 ± 0.117 | 0.994 ± 0.147 | 0.772 ± 0.110 | 1.24 |
| Carbonic anhydrase 3 | CAH3 | 755 | 0.865 ± 0.067 | 0.749 ± 0.092 | 0.865 ± 0.113 | 0.691 ± 0.110 | 1.15 |
| Superoxide dismutase [Cu–Zn] | SODC | 956 | 1.173 ± 0.067 | 1.064 ± 0.064 | 1.106 ± 0.078 | 0.971 ± 0.072 | 1.13 |
| aPhosphoglucomutase-1 | PGM1 | 199 | 1.031 ± 0.052 | 0.807 ± 0.102 | 0.930 ± 0.043 | 0.873 ± 0.049 | 1.10 |
| Spots significantly more abundant in females | |||||||
| Serine protease inhibitor A3K | SPA3K | 139 | 1.098 ± 0.278 | 1.351 ± 0.141 | 0.735 ± 0.057 | 1.031 ± 0.112 | 1.33 |
| Serine protease inhibitor A3L | SPA3L | 168 | 0.958 ± 0.278 | 1.220 ± 0.210 | 0.747 ± 0.144 | 1.282 ± 0.125 | 1.28 |
| aAlpha-2-HS-glycoprotein | FETUA | 169 | 0.978 ± 0.171 | 1.306 ± 0.166 | 0.882 ± 0.192 | 1.308 ± 0.184 | 1.29 |
| Heat shock cognate 71 kDa protein | HSP7C | 53 | 0.932 ± 0.105 | 1.167 ± 0.160 | 1.155 ± 0.096 | 1.329 ± 0.143 | 1.23 |
| Stress-70 protein, mitochondrial | GRP75 | 170 | 0.815 ± 0.068 | 1.006 ± 0.115 | 0.935 ± 0.069 | 1.038 ± 0.099 | 1.17 |
| Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex |
ODP2 | 165 | 0.970 ± 0.043 | 1.138 ± 0.090 | 1.113 ± 0.034 | 1.222 ± 0.065 | 1.12 |
| Beta-enolase | ENOB | 379 | 0.879 ± 0.063 | 1.084 ± 0.104 | 0.870 ± 0.020 | 0.858 ± 0.018 | 1.07 |
| Vimentin | VIME | 260 | 0.941 ± 0.215 | 1.238 ± 0.209 | 0.903 ± 0.140 | 1.209 ± 0.121 | 1.13 |
Protein description and protein ID relate to the Swiss-Prot database entry identified from Mascot searches of CID and ETD MS/MS spectra. Ref# is the gel spot number created during image analysis and is consistent with Fig. 1 and the World-2DPAGE online graphical interface, available at http://world-2dpage.expasy.org/repository/0069/. Relative spot volumes (mean ± SD) are reported for each independent group, i.e. male high-capacity runner (M-HCR), female low-capacity runner (F-LCR) etc. Fold difference values are reported for spots exhibiting significant differences in expression at P < 0.05 at a false discovery rate of <10%.
Contains evidence of post-translational modification: acetylation or phosphorylation (Supplementary Table S1).