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. Author manuscript; available in PMC: 2015 Jun 25.
Published in final edited form as: J Proteomics. 2014 Apr 24;106:230–245. doi: 10.1016/j.jprot.2014.04.015

Table 4.

HCR/LCR strain-specific differences in soleus muscle protein abundance.

Description Protein ID Ref# F-LCR M-LCR F-HCR M-HCR fold
diff.
Respiratory chain
NADH dehydrogenase [ubiquinone] 1 alpha
subcomplex subunit 10, mitochondrial 		NDUAA 535 0.643 ± 0.292 0.721 ± 0.229 1.204 ± 0.302 1.182 ± 0.045 −2.06
ATP synthase subunit alpha ATPA 338 0.956 ± 0.044 1.007 ± 0.098 1.100 ± 0.062 1.132 ± 0.100 −1.08
ATP synthase subunit alpha ATPA 314 0.936 ± 0.048 0.967 ± 0.080 1.154 ± 0.094 1.180 ± 0.085 −1.18
aATP synthase subunit beta ATPB 345 0.861 ± 0.028 0.929 ± 0.059 1.043 ± 0.059 1.012 ± 0.113 −1.10
ATP synthase subunit d ATP5H 861 1.054 ± 0.052 1.134 ± 0.122 1.191 ± 0.080 1.289 ± 0.107 −1.04
Mitochondrial protein import
a60 kDa heat shock protein, mitochondrial CH60 238 1.109 ± 0.112 1.047 ± 0.071 1.258 ± 0.117 1.170 ± 0.044 −1.10
aStress-70 protein, mitochondrial GRP75 107 1.160 ± 0.078 1.061 ± 0.027 1.237 ± 0.070 1.190 ± 0.077 −1.08
aHeat shock cognate 71 kDa protein HSP7C 53 0.932 ± 0.105 1.167 ± 0.160 1.155 ± 0.096 1.329 ± 0.143 −1.11
Pyruvate dehydrogenase complex
Pyruvate dehydrogenase E1 component subunit beta ODPB 635 0.991 ± 0.043 1.018 ± 0.055 1.132 ± 0.026 1.181 ± 0.099 −1.10
Dihydrolipoyllysine-residue acetyltransferase E2
component of pyruvate dehydrogenase complex		 ODP2 165 0.970 ± 0.043 1.138 ± 0.090 1.113 ± 0.034 1.222 ± 0.065 −1.08
Tricarboxylic acid cycle
Fumarate hydratase FUMH 423 0.599 ± 0.304 0.508 ± 0.198 1.458 ± 0.254 1.622 ± 0.335 −3.12
Fumarate hydratase FUMH 425 0.768 ± 0.121 0.822 ± 0.120 1.325 ± 0.177 1.374 ± 0.0115 −1.54
Fumarate hydratase FUMH 426 0.717 ± 0.269 0.599 ± 0.163 1.471 ± 0.282 1.595 ± 0.222 −2.35
Fatty acid metabolism
Very long-chain specific acyl-CoA dehydrogenase ACADV 65 0.878 ± 0.173 0.866 ± 0.121 1.074 ± 0.049 1.074 ± 0.071 −1.32
Enoyl-CoA hydratase ECHM 762 0.890 ± 0.064 0.885 ± 0.078 1.158 ± 0.100 1.026 ± 0.074 −1.20
Amino acid metabolism
3-mercaptopyruvate sulfurtransferase THTM 670 0.899 ± 0.040 0.883 ± 0.047 1.1074 ± 0.079 0.969 ± 0.043 −1.18
b3-hydroxyisobutyrate dehydrogenase 3HIDH 677 1.031 ± 0.065 1.025 ± 0.044 1.185 ± 0.030 1.154 ± 0.134 −1.13
abAspartate aminotransferase, mitochondrial AATM 523 0.809 ± 0.061 0.848 ± 0.072 1.039 ± 0.170 0.922 ± 0.053 −1.16
Ketone body metabolism
Succinyl-CoA:3-ketoacid-coenzyme A transferase 1 SCOT1 164 0.930 ± 0.104 0.947 ± 0.093 1.093 ± 0.051 1.082 ± 0.084 −1.17
Glycolysis
Beta-enolase ENOB 341 1.104 ± 0.033 0.996 ± 0.053 1.146 ± 0.077 1.134 ± 0.099 −1.07
Beta-enolase ENOB 410 2.121 ± 1.562 1.569 ± 0.435 1.017 ± 0.246 0.530 ± 0.247 +2.57
Beta-enolase ENOB 379 0.879 ± 0.063 1.084 ± 0.104 0.870 ± 0.020 0.858 ± 0.018 +1.06
Glyceraldehyde-3-phosphate dehydrogenase G3P 413 1.553 ± 0.374 1.538 ± 0.409 0.679 ± 0.457 0.426 ± 0.082 +2.76
Cytoskeletal
Tripartite motif-containing protein 72 TRI72 261 1.272 ± 0.112 1.143 ± 0.116 0.851 ± 0.229 0.869 ± 0.127 +1.28
Tripartite motif-containing protein 72 TRI72 269 0.655 ± 0.065 0.689 ± 0.091 1.306 ± 0.700 1.2302 ± 0.338 −1.93
Lamin-A LMNA 329 0.979 ± 0.033 1.015 ± 0.061 1.115 ± 0.064 1.139 ± 0.117 −1.07
Miscellaneous
Protein disulphide-isomerase A3 PDIA3 206 1.808 ± 0.506 1.765 ± 1.051 0.424 ± 0.106 0.448 ± 0.070 +2.83
Protein disulphide-isomerase A3 PDIA3 220 1.727 ± 0.426 1.464 ± 0.635 1.084 ± 0.146 0.722 ± 0.125 +1.57
bProtein disulphide-isomerase A3 PDIA3 210 0.774 ± 0.399 0.669 ± 0.265 1.139 ± 0.150 1.146 ± 0.118 −1.75
abProtein NDRG2 NDRG2 433 0.872 ± 0.191 0.985 ± 0.072 1.189 ± 0.186 1.222 ± 0.152 −1.28
abProtein NDRG2 NDRG2 431 0.890 ± 0.197 0.920 ± 0.102 1.165 ± 0.092 1.098 ± 0.127 −1.37
Serine protease inhibitor A3K SPA3K 123 1.159 ± 0.316 1.435 ± 0.261 0.777 ± 0.130 1.008 ± 0.246 +1.53
Serine protease inhibitor A3K SPA3K 139 1.098 ± 0.278 1.351 ± 0.141 0.735 ± 0.057 1.031 ± 0.112 +1.43
Voltage-dependent anion-selective channel protein 1 VDAC1 385 1.317 ± 0.320 1.245 ± 0.249 0.944 ± 0.128 0.830 ± 0.191 +1.33
Voltage-dependent anion-selective channel protein 1 VDAC1 650 0.945 ± 0.086 0.973 ± 0.090 1.102 ± 0.119 1.166 ± 0.035 −1.16
Aspartyl-tRNA synthetase, cytoplasmic SYDC 156 0.831 ± 0.103 0.928 ± 0.079 1.022 ± 0.059 1.084 ± 0.111 −1.19
Aldehyde dehydrogenase, mitochondrial ALDH2 337 1.058 ± 0.079 1.013 ± 0.102 1.176 ± 0.100 1.253 ± 0.063 −1.16
Creatine kinase M-type KCRM 541 0.738 ± 0.242 0.853 ± 0.238 1.275 ± 0.227 1.303 ± 0.279 −1.79
Hypoxanthine-guanine phosphoribosyltransferase HPRT 757 0.768 ± 0.138 0.822 ± 0.052 1.060 ± 0.082 0.874 ± 0.114 −1.31
Serine protease inhibitor A3L SPA3L 539 1.557 ± 0.248 1.688 ± 0.395 0.762 ± 0.657 0.311 ± 0.055 +3.18

Protein description and protein ID relate to the Swiss-Prot database entry identified from Mascot searches of CID and ETD MS/MS spectra. Ref# is the gel spot number created during image analysis and is consistent with Fig. 1 and the World-2DPAGE online graphical interface, available at http://world-2dpage.expasy.org/repository/0069/. Relative spot volumes (mean ± SD) are reported for each independent group, i.e. male high-capacity runner (M-HCR), female low-capacity runner (F-LCR) etc. Fold difference values are reported for spots exhibiting significant differences in expression at P < 0.05 at a false discovery rate of <10%.

a

Present in Table S3 from {Bell 2009} containing genes from the longevity interactome (core or primary interactors) that are differentially expressed in young versus old human muscle.

b

Contains evidence of post-translational modification: acetylation or phosphorylation (Supplementary Table S1).