. 2014 Feb 25;32:83–101. doi: 10.3767/003158514X679984

Table 2.

Primers used and alternative new primers designed for current study and optimised PCR protocols.

Locus	Primers	Optimised PCR protocols	Approximate sizes of the PCR amplicons obtained	References for primers^a & protocols^b
ACT	ACT-512F:	(95 °C: 30 s, 55 °C: 50 s, 72 °C: 1 min)	280bp (ACT512F/ACT783R)	Carbone & Kohn 1999^a
	ATGTGCAAGGCCGGTTTCGC	×39 cycles for ACT512F/ACT783R
	ACT-783R	(95 °C: 30 s, 58 °C: 50 s, 72 °C: 1 min)	380bp (ACT512F/ACT878R)	This study^a,^b
	(5’-TACGAGTCCTTCTGGCCCAT-3’)	×39 cycles for ACT512F/ACT878R
	ACT878R (new):
	ATCTTCTCC ATGTCGTCCCAG
TUB	Bt-2a:	(95 °C: 30 s, 58 °C: 50 s, 72 °C: 1 min)	500bp	Glass & Donaldson 1995^a
	GGTAACCAAATCGGTGCTGCTTTC	×39 cycles		Udayanga et al. 2012a^b
	BT-2b:
	ACCCTCAGTGTAGTGACCCTTGGC
CAL	CAL-228F:	(95 °C: 30 s, 55 °C: 50 s, 72 °C: 1 min)	500bp (CAL228F.CAL737R)	Carbone & Kohn 1999^a
	GAGTTCAAGGAGGCCTTCTCCC	×39 cycles for CAL228F/CAL737R
	CAL-737R:	(95 °C: 30 s, 52 °C: 50 s, 72 °C: 1 min)	800bp (CL1/CL2A)	O’Donnell et al. 2000^a;
	CATCTTCTGGCCATCATGG	×39 cycles for CL1/CL2A		Udayanga et al. 2012a^b
	CL1F:	(95 °C: 30 s, 51 °C: 50 s, 72 °C: 1 min)	570bp (CAL563F/CL2A)	This study^a,^b
	GARTWCAAGGAGGCCTTCTC	×39 cycles for CAL563F/CL2A
	CL2A :
	TTTTTGCATCATGAGTTGGAC
	CAL563F (new):
	GACAAATCA CCACCAARGAGC
EF1-α	EF1-728F:	(95 °C: 30 s, 58 °C: 50 s, 72 °C: 1 min)	350bp	Carbone & Kohn 1999^a
	CATCGAGAAGTTCGAGAAGG	×39 cycles		Udayanga et al. 2012a^b
	EF1-986R:
	TACTTGAAGGAACCCTTACC
ITS	ITS1:	(95 °C: 30 s, 55 °C: 50 s, 72 °C: 1 min)	600bp	White et al. 1990^a
	TCCGTAGGTGAACCTGCGG	×39 cycles		Udayanga et al. 2012a^b
	ITS4:
	TCCTCCGCTTATTGATATGC