Expression levels of lamin b1 from SCN, kidney, and liver extracts in C57BL/6J mice (A and B). (A) mRNA levels of Lmnb1 and Gapdh were assayed at indicated circadian times (CT, n = 4). (B) Representative immunoblots show the levels of LMNB1, GAPDH, and β-ACTIN. (C) Representative immunoblots show PER2 (with intensity values indicated at the bottom) and LMNB1 abundance in Lmnb1+/Δ, wild-type and LMNB1BAC liver extracts. (D) Expression patterns of LMNB1, LBR, and MAN1 in C57BL/6 mouse livers at indicated Zeitgeber times (ZT) (n = 3). Quantifications (top panels) of Western blots (bottom panel) were obtained by using GAPDH as a loading control. Data represent means ± SD.
DOI:
http://dx.doi.org/10.7554/eLife.02981.003