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(A) Reduction of MAN1 transcripts (13 nM siRNA) reduced Bmal1 promoter activity (n = 3, *p < 0.001). (B) Over-expression of FLAG-tagged MAN1 (F-MAN1) enhanced Bmal1-Luc activity (n = 3, *p < 0.001). (C) Over-expression of FLAG-tagged MAN1 (F-MAN1) enhanced luciferase activities driven by mBmal1 promoter (530 bp) or hBMAL1 promoter (3.4 kb) (n = 3, *p < 0.05). Error bars represent SD.
