Figure 2.

Target area (a), DNA methylation levels (b), and mRNA levels (c) of the fatty acid binding protein 4 (FABP4) gene in intramuscular fat (IMF) and the muscle portion of Korean cattle steer longissimus dorsi muscle tissue. (a) DNA methylation assay target area in the FABP4 gene promoter region and transcription factor binding sites. HSF2, heat shock factor 2; CdxA, caudal-related homeobox A; AML-1a, acute myeloid leukemia-1a. (b) DNA methylation levels were determined by pyrosequencing bisulfite-treated DNA. Values are the mean+SE (n = 5). (c) The mRNA levels were determined by real-time PCR and normalized with a housekeeping gene. Muscle portion data were normalized to 1.0. Values are the mean+SE (n = 10). *** p<0.001. PCR, polymerase chain reaction; SE, standard errors.