Table 2.

Primer sequences used in bisulfite pyrosequencing

Gene name (Symbol)	Accession number	Primer	PCR primer sequence (5′-3′)	Sequencing primer (5′-3′)	Target region: relative to transcription start site	Number of CpG sites checked
Peroxisome proliferator-activated receptor gamma isoform 1 (PPARG1)	NM_181024	Forward	tgaggtttgtggtgatgattattt	aacccaaataaataaaattct	+144 to +225	3
		Reverse	aacacaatttccccaaccatta
Fatty acid binding protein 4 (FABP4)	BT10868	Forward	tttaatttttttgttaggaattgggttat	gttaggaattgggttatatagta	−9,664 to −9,469	5
		Reverse	aaaaacatacaacctaaatcccttaca

PCR, polymerase chain reaction.