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PCR primers for real-time PCR assay
| Target species | Forward | Primer sequence |
|---|---|---|
| Total bacteria1 | F | CGGCAACGAGCGCAACCC |
| R | CCATTGTAGCACGTGTGTAGCC | |
| Fungi1 | F | GAGGAAGTAAAAGTCGTAACAAGGTTTC |
| R | CAAATTCACAAAGGGTAGGATGATT | |
| R. flavefaciens1 | F | CGAACGGAGATAATTTGAGTTTACTTAGG |
| R | CGGTCTCTGTATGTTATGAGGTATTACC | |
| F. succinogenes1 | F | GTTCGGAATTACTGGGCGTAAA |
| R | CGCCTGCCCCTGAACTATC | |
| Protozoa2 | F | GCTTTCGWTGGTAGTGTATT |
| R | CTTGCCCTCYAATCGTWCT | |
| R. albus3 | F | CCCTAAAAGCAGTCTTAGTTCG |
| R | CCTCCTTGCGGTTAGAACA |
PCR, polymerase chain reaction.
