| Component | Amount per reaction (μl) | Final |
|---|---|---|
| 5xPCR buffer | 5 | 1x |
| 5 M Betaine | 5 | 1 M |
| 10 mM dNTPs | 0.5 | 200 nM |
| DNA template | 20 ng | 20 ng |
| H2O | to 25 μl | |
| Proof-reading polymerase (2U/μl) | 0.5 | 1U |
| 1 μM each gene-specific Fw + Rv primers | 2.5 each | 100 nM each |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.