| Component | Amount per reaction (μl) | Final |
|---|---|---|
| single-fly DNA template | 1 | |
| Fw + Rv primers (10 μM each) | 0.5 each | 250 nM |
| dNTPs (2 mM) | 1.5 | 150 nM |
| GoTaq buffer (5x) | 4 | 1x |
| GoTaq polymerase (5U/μl) | 0.1 | 0.5U |
| H2O | 12.4 |
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