Figure 2.

Effect of blockage and site of block retrieval on quality control parameters for MiSeq. (A) Distribution of DNA-QC scores among samples run on the MiSeq. (B) The effect of blockage on QC scores, showing the best QC scores for fresh tissue obtained in 2013. (C) A plot of QC scores by referring hospital shows that sufficient quality tumour DNA can be extracted from nearly all submitted tumour blocks despite varying laboratory practices. Abbreviations: DNA=deoxyribonucleic acid; QC=quality control.