Figure 1.
Expression of HOXB9 in colon adenocarcinoma cell lines and patients. (A) HOXB9 expression in Wooster Cell line data set of oncomine: colorectal cancer cells displayed the highest expression of HOXB9 among 19 types of cancer cell lines included. (B) HOXB9 expression in Bittner Multi-cancer data set of oncomine: colorectal cancer showed the highest expression of HOXB9 in patients among 17 different types of cancer. (C) Left panel: HOXB9 protein levels in colon adenocarcinoma cell lines; right panel: HOXB9 protein levels in breast cancer cell lines. Protein levels of HOXB9 in cell lines were analysed by western blot probed by an anti-HOXB9 monoclonal antibody. Actin was used as loading control. (D) HOXB9 expression in normal colon, intestine and duodenum in mouse. Mouse colon, intestine and duodenum were dissected and tissues were homogenised and quantified. Equal amount of each tissue was used for western blot analysis. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control.