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Overexpression and purification of M. tuberculosis PanD in E. coli. Lane 1–4: PanD protein, which appears as a 16-kD band, eluted by 50, 100, 200, 400 mM imidazole, respectively. Lane 5: Protein molecular weight marker (116, 66, 45, 35, 25, 18, 14 kD).
