Table 1.

Pore lining amino residues of selected AQPs.

Region	hAQP1	hAQP6	hAQP5	GlpF	hAQP9	AtNIP2;1	LpGlpF1	LpGlpF4	SmAQP
TM1	F	Y	F	L	L	L	M	M	L
	V	V	V	I	I	I	I	I	M
	S	G	G	G	G	G	G	G	G
	I	V	L	V	C	C	V	V	S
	A	V	A	V	V	I	H	H	L
TM2	V	I	I	I	I	I	A	A	V
	F	F	F	W	F	W	W	W	W
	I	T	I	V	V	I	I	I	V
	A	V	A	I	I	V	L	L	V
Half helix B	G	G	G	G	G	S	N	N	S
	A	A	G	A	G	A	V	V	G
	L	A	I	L	I	F	I	I	C
	N	N	N	N	N	N	N	N	N
	V	V	I	V	V	V	M	M	V
TM4	L	L	L	L	L	L	F	F	L
	V	V	I	I	V	V	I	I	A
TM5	A	P	P	A	E	E	V	V	H
	I	I	I	I	I	I	V	V	L
	V	V	V	I	I	V	V	V	V
	H	H	H	G	A	V	G	G	V
	I	I	I	G	G	G	G	G	A
Half helix E	G	G	G	G	G	G	G	G	G
	C	C	C	F	C	A	F	F	A
	G	S	S	A	A	S	A	A	A
	I	M	M	M	M	M	M	M	L
	N	N	N	N	N	N	N	N	N
	R	R	R	R	R	R	R	R	R
	S	P	P	P	P	P	P	P	P
Positive (H,R)	2	2	2	1	1	1	2	2	2
Polar (S,T,Y,N)	3	5	3	2	2	4	3	3	4

Residues of the constriction sites are highlighted (ar/R region: bold, upright characters; NPA region: slanted letters).