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Effect of pH on the tyrosinase activity of the crude extract of A. bisporus. Data were obtained as mean value of optical density. Assays were done at 35°C and the activity of the sample was incubated on 100 mM acetate buffer at 4.0-5.0 pH, 100 mM phosphate buffer at 6.0–8.0 pH, and 100 mM Tris-HCl buffer at 9.0-10 pH. The optimum activity of the sample at pH 6.0 was taken as 100%.
