Figure 7.

Effect of the seven supplement's components on HaCaT keratinocyte IL-6 production. Cells were exposed for 18 h to increasing concentrations of the components in the absence (a) or in the presence (b) of TNF-α (20 ng/mL, 6 h pretreatment and further 18 h with the components); the two concentrations used for each component are the lowest and highest concentrations reported in Section 2. Data are the means ± SE of 4 determinations. a: significantly different from control (P < 0.05); b: significantly different from control (P < 0.01), one-way ANOVA followed by Dunnett's test. (c) Cells were treated simultaneously with all the components, both in basal conditions or in the presence of TNF-α (20 ng/mL), following the timing of the previous experiment. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of 4 determinations. ∗: significantly different from respective control (P < 0.0001, two-tailed unpaired t-test).