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Mutational spectra of six different PCR enzymes are presented. Results with Taq polymerase are mutations observed in sequencing clones from two independent PCR reactions. For indel mutations, the type of insertion or deletion is indicated.
| Taq | AccuPrime-Taq, HF | KOD | Pfu | Phusion | Pwo | |
|---|---|---|---|---|---|---|
| Transitions | ||||||
| A•T→G•C | 67 | 12 | 9 | 3 | 6 | 6 |
| G•C→A•T | 28 | 5 | 5 | 5 | 5 | 4 |
| Transversion | ||||||
| A•T→T•A | 1 | 1 | 1 | 1 | ||
| A•T→C•G | 2 | |||||
| G•C→T•A | 3 | |||||
| Indels | 1 (T del.) |
1 (T del.) |
2 (A ins., TCT del in (TCT)5 run) |
Insertion = ins.; deletion = del.
