Fig. 2.

Dependence of analyte migration time through the nano ES capillary on the applied pressure to the sample chamber (A): the migration time of analytes was calculated by Hagen–Poiseuille equation. Electrophoretic net mobilities of analytes under comparable conditions as during the nano ES process (B): the EOF mobility is increasing with increasing pH values, whereas the electrophoretic net mobilities of analytes do not change significantly.