Table 2.
Statistic classification of related proteins in NRK-52E cell lines with Fe3O4 NPs treatment.
| UNIPROT accessiona | UNIPROT accession numbera |
Protein identification | Molecular mass (kDa)b | Number of peptidesb | Ratioc |
|---|---|---|---|---|---|
| Cell death and apoptosis related proteins | |||||
| APAF_RAT | Q9EPV5 | Apoptotic protease-activating factor 1 | 146.1 | 2 | 780.9 |
| PDC10_RAT | Q6NX65 | Programmed cell death protein 10 | 25.0 | 2 | 233.2 |
| LEG1_RAT | P11762 | Galectin-1 | 15.5 | 5 | 1.75 |
| PDC6I_RAT | Q9QZA2 | Programmed cell death 6-interacting protein | 99.2 | n.d. | n.d. |
|
| |||||
| Ras-related proteins | |||||
| RAB7L_RAT | Q63481 | Ras-related protein Rab-7L1 | 23.7 | 2 | 3.2 |
| RAB1A_RAT | Q6NYB7 | Ras-related protein Rab-1A | 23.4 | 13 | 2.3 |
| RAB2A_RAT | P05712 | Ras-related protein Rab-2A | 24.1 | 3 | 1.6 |
| RAC1_RAT | Q6RUV5 | Ras-related C3 botulinum toxin substrate 1 | 22.4 | 2 | 1.2 |
| RAP1A_RAT | P62836 | Ras-related protein Rap-1A | 21.8 | n.d. | n.d. |
| RAB7A_RAT | P09527 | Ras-related protein Rab-7a | 24.3 | n.d. | n.d. |
|
| |||||
| Glutathione related proteins | |||||
| GSHR_RAT | P70619 | Glutathione reductase (Fragment) | 47.8 | 2 | 3.1 |
| GSTM1_RAT | P04905 | Glutathione S-transferase Mu 1 | 26.7 | 2 | 2.8 |
| GSTM2_RAT | P08010 | Glutathione S-transferase Mu 2 | 26.5 | 3 | 2.4 |
| GSTP1_RAT | P04906 | Glutathione S-transferase P | 24.1 | 6 | 1.5 |
| GSTA3_RAT | P04904 | Glutathione S-transferase alpha-3 | 25.9 | 4 | 1.2 |
|
| |||||
| Chaperone proteins | |||||
| HSBP1_RAT | Q8K3X8 | Heat shock factor-binding protein 1 | 8.7 | 2 | 2.8 |
| HS90B_RAT | P34058 | Heat shock protein HSP 90-beta | 86.0 | 32 | 2.7 |
| SERPH_RAT | P29457 | Serpin H1 | 47.7 | 30 | 2.5 |
| HS90A_RAT | P82995 | Heat shock protein HSP 90-alpha | 87.7 | 31 | 2.4 |
| HSP74_RAT | O88600 | Heat shock 70 kDa protein 4 | 97.0 | 2 | 2.2 |
| HSP7C_RAT | P63018 | Heat shock cognate 71 kDa protein | 72.8 | 40 | 1.9 |
| PDIA4_RAT | P38659 | Protein disulfide-isomerase A4 | 75.1 | 9 | 1.8 |
| CH60_RAT | P63039 | 60 kDa heat shock protein, mitochondrial | 62.6 | 29 | 1.6 |
| CH10_RAT | P26772 | 10 kDa heat shock protein, mitochondrial | 11.2 | 3 | 1.6 |
| GRP78_RAT | P06461 | 78 kDa glucose-regulated protein | 74.4 | 19 | 1.6 |
| ENPL_RAT | Q66HD0 | Endoplasmin | 95.4 | 17 | 1.6 |
| PDIA1_RAT | P04785 | Protein disulfide-isomerase | 59.1 | 24 | 1.6 |
| ERP29_RAT | P52555 | Endoplasmic reticulum resident protein 29 | 29.3 | 3 | 1.6 |
| PDIA6_RAT | Q63081 | Protein disulfide-isomerase A6 | 49.6 | 4 | 1.5 |
| PDIA3_RAT | P11598 | Protein disulfide-isomerase A3 | 58.6 | 17 | 1.3 |
aProtein accession and protein accession number were received from the UNIPROT database available online: http://www.uniprot.org/uniprot (accessed on May 25, 2014).
bMolecular weight and number of protein peptides according to the Swiss-Prot database in the Mascot search engine.
cRatio values of each protein according to protein list in the Mascot Distiller.