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. Author manuscript; available in PMC: 2015 Jul 31.
Published in final edited form as: Cell. 2014 Jul 24;158(3):481–491. doi: 10.1016/j.cell.2014.06.022

Figure 1. Neutralization activity of CH103 clonal lineage antibodies against autologous CH505 viruses.

Figure 1

Heat map analysis of neutralization data generated from 124 pseudoviruses (row) and 13 CH103 lineage mAbs (column). The neutralization potency (IC50) is shown in different shades of colors as indicated in the histogram; from white (>50µg/ml) to dark red (0.079 µg/ml). The Env pseudoviruses were generated for the CH505 T/F virus and variants from weeks 4 – 100 and were all assayed against the unmutated common ancestor (UCA), intermediate antibodies (IA8-1) and mature bnAbs (CH103-106) in the TZM-bl cell-based neutralization assay. The resistant viruses (blue brackets) were defined as those with the average IC50 values 1.8 fold high than that of the T/F pseudoviruses for later IAs (IA3-IA1) and all mature Abs. The week 30 viruses that fully escaped from the early IAs (IA8-IA4) are also indicated by blue bracket. See also Table S1; Figures S1 and S4.