Figure 5. Neutralization activity of CH235 clonal lineage antibodies against CH505 Env loop D mutants.
Heat map analysis of neutralization data generated from 10 loop D mutants (row) and 10 CH235 lineage mAbs (column). The neutralization potency (IC50) is shown in different shades of colors as indicated in the histogram; from white (>50 µg/ml) to dark red (0.1 µg/ml). The CH505 T/F and loop D mutant Env pseudoviruses were assayed against the unmutated common ancestor (UCA), intermediate antibodies (IA4-1) and mature autologous neutralizing antibodies (CH235, CH236, CH239, CH240 and CH241) in the TZM-bl cell-based neutralization assay. The partially and fully resistant viruses are indicated by the blue bracket. See also Table S5.
