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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Glia. 2014 Jun 26;62(10):1699–1712. doi: 10.1002/glia.22709

Figure 2.

Figure 2

ProNGF fails to induce apoptosis in primary vestibular schwannoma cultures. A. Primary VS cultures immunostained with anti-p75NTR antibody (green). Nuclei are labeled with DAPI (blue). B,C. Primary human VS cultures were treated with escalating doses of proNGF, fixed, immunostained with anti-S100 (green) antibodies and labeled with TUNEL (red) and DAPI (blue). D. The average percent of TUNEL-positive, S100-positive condensed nuclei from primary human vestibular schwannoma cultures or non-neoplastic mouse Schwann cell cultures were scored and plotted relative to cultures not treated with proNGF (control). Error bars present standard error of the mean. Vestibular schwannoma data are from cultures derived from six separate patients while data for non-neoplastic Schwann cells were derived from 3 separate cultures. There was no significant difference between treatment conditions for the vestibular schwannoma cultures by one way ANOVA. *p<0.01 by Student's two tailed t-test.