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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Glia. 2014 Jun 26;62(10):1699–1712. doi: 10.1002/glia.22709

Figure 3.

Figure 3

Primary vestibular schwannoma cultures express sortilin and RIP2. A. Western blot of protein lysates from human GAN and human VS specimens probed with anti-sortilin antibodies. The blots were stripped and reprobed with anti-Rho-GDI antibodies to compare protein loading. Blots present the lysates with the highest and lowest sortilin expression compared with Rho-GDI. Bots for lysates from 3 additional tumors demonstrated similar expression levels (not shown). B-D. Immunostaining of primary VS cultures with anti-p75NTR (B, green) and anti-sortilin (C, red) antibodies (D, combined). Nuclei were labeled with DAPI (blue). E. Western blot of protein lysates from rat SC and primary human VS cultures probed with anti-RIP2 antibodies. Blots present the lysates with the highest and lowest sortilin expression compared with b-actin. Bots for lysates from 2 additional cultures from separate tumors demonstrated similar expression levels (not shown).