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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Glia. 2014 Jun 26;62(10):1699–1712. doi: 10.1002/glia.22709

Figure 5.

Figure 5

ProNGF activates JNK and NF-κB in primary VS cultures. A. Western blots of protein lysates from primary VS cultures treated Ad5.empty vector (Ad.EV) or Ad5.merlin (Ad.merlin) and probed with anti-phosphorylated JNK antibodies. The blots were stripped and reprobed with non phospho-specific anti-JNK. Parallel blots were probed with anti-merlin and subsequently with anti-b-actin antibodies.B. Western blots of protein lysates from primary VS cultures treated with proNGF and probed with anti-phosphorylated JNK. The blots were stripped and reprobed with non phospho-specific anti-JNK and subsequently with anti-phosphorylated c-JUN antibodies. C&D. Average NF-κB activity, relative to control, in primary VS cultures treated with SP600125 (SP) or proNGF or transfected with scrambled or JNK1/2 specific siRNA oligonucleotides. Data in B are from cultures derived from six separate patients and in C from four separate patients. *p<0.05, **p<0.01 by one way ANOVA followed by Dunn's method.