Table 4.
PCR primers for rs76719109 and rs592297 AEI assay
| Assay | DNA | Primer Sense | Primer Sequence |
|---|---|---|---|
| rs76719109 |
cDNA |
Sense |
5′CCATCTCATCCCTGCGTGTCTCCGACTCAGxxxxxTGGAGTCAACCAGGTGAAAA |
| |
cDNA |
Anti-sense |
5′ CCTCTCTATGGGCAGTCGGTGATTTGGTTGCGTCATTACAGGA |
| |
gDNA |
Sense |
5′CCATCTCATCCCTGCGTGTCTCCGACTCAGxxxxxTGGAGTCAACCAGGTGAAAA |
| |
gDNA |
Anti-sense |
5′ CCTCTCTATGGGCAGTCGGTGATAGGAGCTTTTTCAACTCACCA |
| rs592297 |
cDNA |
Sense |
5′CCATCTCATCCCTGCGTGTCTCCGACTCAGxxxxxTGAACACAGAAAAACTCCTAAAAA |
| |
cDNA |
Anti-sense |
5′ CCTCTCTATGGGCAGTCGGTGATGGCAGCATTTATTACCCCATT |
| |
gDNA |
Sense |
5′CCATCTCATCCCTGCGTGTCTCCGACTCAGxxxxxTGAACACAGAAAAACTCCTAAAAA |
| gDNA | Anti-sense | 5′ CCTCTCTATGGGCAGTCGGTGATTCTGTGAAAACTTGAGGTTAAAAA |
"xxxxx" denotes 5 nucleic acid barcode, which is unique for each individual. Note that gDNA and cDNA were amplified by the same barcoded forward primers. Genomic DNA and cDNA were differentiated by the reverse primers.