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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Surgery. 2014 Jun 2;156(3):548–555. doi: 10.1016/j.surg.2014.04.014

Figure 1. CCN2 mRNA and protein are present in mouse HSC exosomes.

Figure 1

(A) Quantitative RT-PCR of CCN2 mRNA in exosomes isolated from primary mouse HSC on Day 3-14 of culture. Data are from three independent experiments and expressed as mean ± s.e.m. *P<0.05 as determined by Student’s t-test using SIGMA PLOT 11.0 software (SPSS Inc., Chicago, IL). (B) Western blot for CCN2 in exosomes isolated from 24-hour conditioned medium from individual T-75 flasks containing primary mouse HSC on days 3-9 of culture (5 g total exosomal protein/lane). (C) CCN2 Western blot of exosomes isolated from P5 mouse HSC. Data are representative of three independent determinations.

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