. Author manuscript; available in PMC: 2015 Sep 1.

Published in final edited form as: J Struct Biol. 2014 Aug 1;187(3):223–235. doi: 10.1016/j.jsb.2014.07.006

Table 2. Characteristics of structures containing linear cyto-GspL arrangements.

Structure		Vv #3	Vv #1 and #2	Vc N1E•cyto-GspL	Vc cyto-GspL
Crystallization Condition		0.2M Na Malonate pH7. 18% PEG335 1 mM AMPPNP 2 mM MgCl₂		0.2M CaOAc₂ 0.1M Bis-Tris-HCl pH 5.75 15-20% PEG3350	0.15M CaOAc₂ 0.1M Tris-HCl pH 8 11-15% PEG6000
Space Group		C2	C2	P2	C2
α-interface	BSA (Å²)	878	960	1150	1102
	r.m.s.d.	-	0.10	1.23	1.43
	Kappa	-	4.1	19.1	16.0
β-interface	BSA (Å²)	521	528	679	766
	r.m.s.d.	-	0.17	1.23	1.43
	Kappa	-	4.1	19.1	16.0

Vv #3: cyto-GspL of V. vulnificus GspE•cyto-GspL #3 complex; Vv #1 and #2: cyto-GspL of V. vulnificus GspE•cyto-GspL #1and #2 complex; Vc N1E•cyto-GspL: cyto-GspL of V. cholerae N1E•cyto-GspL (PDB 2BH1) (Abendroth et al., 2005); Vc cyto-GspL: V. cholerae cyto-GspL (PDB 1YF5) (Abendroth et al., 2004a).

R.m.s.d. and rotation angle (kappa) calculations are based on a superposition of cyto-GspL from three different structures to the cyto-GspL of V. vulnificus GspE•cyto-GspL #3 complex. The resultant superposition operation is applied to the entire cyto-GspL•cyto-GspL pairs. The second cyto-GspL in the pairs is subsequently superimposed on the cyto-GspL related to Vv #3 by a crystallographic twofold. The r.m.s.d. and interdomain angle of this second superposition are given in the Table.