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. 2014 Jun 30;42(15):e117. doi: 10.1093/nar/gku553

Figure 1.

Figure 1.

Method for the site-directed spin labeling approach. (A) Schematic representation of the synthesis of site-specific spin labeled RNA. 6-thioguanosine-5′-O-monophosphate (pG6T = 6-T-GMP) is incorporated at a specific position. (B) The proxyl-RNA coupling reaction involves the addition of 3-(2-Iodoacetamido)-proxyl on 6-thioguanosine-5′-O-monophosphate. (C) Secondary structure of the 55-nt (tyrS) RNA molecule corresponding to the specifier loop domain and K-turn sequence motif of the tyrS leader RNA. The Specifier Loop Domain and the tetraloop are boxed. The arrow indicates the chosen segmentation site and the circle indicates the thio-modified site.