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Characterization of spin-labeled RNA by EPR spectroscopy. EPR spectra of covalently labeled RNA (bottom) compared to free PROXYL (top). Both spectra were recorded in 70 mM sodium phosphate buffer (pH = 6.5), supplemented by 10% v/v D2O, and acquired with identical instrumental parameters. In this case the concentration of spin-labeled RNA was found to be 150 ± 15 μM, whereas the concentration of the free proxyl spectrum corresponds to 50 μM.
