Table 2. Characteristics of deletable and undeletable chromosomal regions.
| Chromosome (length) | Straina | Deleted regionb | Region namec | Deletion length (kb) | No. of genes (n) | No. of transformants obtained | No. of transformants examined for karyo-type | Transformants with expected deletion | Frequencyd | Deletable/ Undeletable | References/ remarks |
|---|---|---|---|---|---|---|---|---|---|---|---|
| I (230 kb) | ScΔ(Chr 1-1) | 1–51 910 | Chr 1-1 | 51.9 | 30 | 75 | 20 | 12 | 60.00% | Deletable | This study |
| 109 298–138 358 | Chr 1-2 | 29.1 | 19 | 55 | 29 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 1-3) | 178 545–230 218 | Chr 1-3 | 51.7 | 33 | 145 | 20 | 16 | 80.00% | Deletable | This study | |
| II (813 kb) | ScΔ(Chr 2-1) | 1–10 856 | Chr 2-1 | 10.8 | 18 | 118 | 28 | 26 | 92.85% | Deletable | This study |
| 21 866–37 346 | Chr 2-2 | 15.5 | 14 | 48 | 25 | 0 | 0.00% | Undeletable | This study | ||
| 92 578–121 720 | Chr 2-3 | 29.2 | 18 | 63 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 205 408–220 469 | Chr 2-4 | 15.0 | 9 | 64 | 28 | 0 | 0.00% | Undeletable | This study | ||
| 265 907–292 296 | Chr 2-5 | 26.3 | 15 | 75 | 28 | 0 | 0.00% | Undeletable | This study | ||
| 318 749–330 960 | Chr 2-6 | 12.2 | 9 | 71 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 577 219–602 500 | Chr 2-7 | 25.3 | 19 | 49 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 650 769–681 129 | Chr 2-8 | 30.4 | 22 | 56 | 20 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 2-9) | 744 846–813 184 | Chr 2-9 | 68.3 | 38 | 78 | 20 | 4 | 20.00% | Deletable | This study | |
| III (316 kb) | ScΔ(Chr 3-1) | 1–18 822 | Chr 3-1 | 18.8 | 20 | 163 | 20 | 18 | 90.00% | Deletable | This study |
| 68 950–83 624 | Chr 3-2 | 14.7 | 13 | 66 | 21 | 0 | 0.00% | Undeletable | This study | ||
| 116 424–133 170 | Chr 3-3 | 16.7 | 12 | 99 | 21 | 0 | 0.00% | Undeletable | This study | ||
| 145 265–186 097 | Chr 3-4 | 40.8 | 31 | 51 | 21 | 0 | 0.00% | Undeletable | This study | ||
| 246 961–268 191 | Chr 3-5 | 21.2 | 17 | 70 | 25 | 0 | 0.00% | Undeletable | This study | ||
| IV (1532 kb) | ScΔ(Chr 4-1) | 1–29 985 | Chr 4-1 | 29.9 | 17 | 198 | 20 | 20 | 100.00% | Deletable | This study |
| ScΔ(Chr 4-2) | 119 330–159 986 | Chr 4-2 | 40.6 | 24 | Deletable | Ueda et al. (17) | |||||
| 239 739–258 679 | Chr 4-3 | 18.9 | 13 | 59 | 21 | 0 | 0.00% | Undeletable | This study | ||
| 309 991–334 228 | Chr 4-4 | 24.2 | 16 | 52 | 21 | 0 | 0.00% | Undeletable | This study | ||
| 494 271–521 840 | Chr 4-5 | 27.6 | 24 | 87 | 25 | 0 | 0.00% | Undeletable | This study | ||
| 584 190–603 837 | Chr 4-6 | 19.7 | 14 | 54 | 21 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 4-7) | 632 193–676 133 | Chr 4-7 | 44.0 | 29 | Deletable | Ueda et al. (17) | |||||
| 691 278–730 916 | Chr 4-8 | 39.7 | 20 | 50 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 958 372–998 900 | Chr 4-9 | 40.6 | 31 | 88 | 20 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 4-10) | 1 473 549–1 487 620 | Chr 4-10 | 14.1 | 10 | 55 | 20 | 12 | 60.00% | Deletable | This study | |
| ScΔ(Chr 4-11) | 1 502 766–1 531 933 | Chr 4-11 | 29.2 | 19 | 106 | 20 | 20 | 100.00% | Deletable | This study | |
| V (577 kb) | ScΔ(Chr 5-1) | 1–38 703 | Chr 5-1 | 38.7 | 23 | 73 | 24 | 16 | 66.66% | Deletable | This study |
| 55 219–80 675 | Chr 5-2 | 25.4 | 22 | 90 | 20 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 5-3) | 126 605–141 203 | Chr 5-3 | 14.6 | 16 | 52 | 20 | 8 | 40.00% | Deletable | This study | |
| 260 306–303 330 | Chr 5-4 | 43.0 | 27 | 95 | 25 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 5-5) | 541 219–576 874 | Chr 5-5 | 35.6 | 28 | Deletable | Sugiyama et al. (14) | |||||
| VI (270 kb) | ScΔ(Chr 6-1) | 1–36 314 | Chr 6-1 | 36.3 | 24 | 95 | 20 | 14 | 70.00% | Deletable | This study |
| 161 023–196 416 | Chr 6-2 | 35.4 | 23 | 59 | 22 | 0 | 0.00% | Undeletable | This study | ||
| VII (1091 kb) | 1–32 647 | Chr 7-1 | 32.6 | 20 | 69 | 26 | 0 | 0.00% | Undeletable | This study | |
| 82 711–95 454 | Chr 7-2 | 12.7 | 11 | 73 | 25 | 0 | 0.00% | Undeletable | This study | ||
| 129 738–171 415 | Chr 7-3 | 41.7 | 23 | 56 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 190 867–210 017 | Chr 7-4 | 19.2 | 12 | 68 | 22 | 0 | 0.00% | Undeletable | This study | ||
| 555 493–579 150 | Chr 7-5 | 23.6 | 19 | 88 | 28 | 0 | 0.00% | Undeletable | This study | ||
| 809 906–835 553 | Chr 7-6 | 25.6 | 21 | 58 | 23 | 0 | 0.00% | Undeletable | This study | ||
| 939 364–974 120 | Chr 7-7 | 34.8 | 21 | 77 | 27 | 0 | 0.00% | Undeletable | This study | ||
| 1 055 720–1 090 940 | Chr 7-8 | 35.2 | 22 | 60 | 28 | 0 | 0.00% | Undeletable | This study | ||
| VIII (562 kb) | 1–71 481 | Chr 8-1 | 71.4 | 47 | 97 | 25 | 0 | 0.00% | Undeletable | This study | |
| 79 586–111 810 | Chr 8-2 | 32.3 | 28 | 86 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 162 591–176 986 | Chr 8-3 | 14.4 | 11 | 63 | 21 | 0 | 0.00% | Undeletable | This study | ||
| 196 188–214 563 | Chr 8-4 | 18.3 | 17 | 79 | 25 | 0 | 0.00% | Undeletable | This study | ||
| 358 040–383 752 | Chr 8-5 | 25.7 | 28 | 88 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 397 528–421 202 | Chr 8-6 | 23.7 | 13 | 49 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 502 800–562 643 | Chr 8-7 | 59.8 | 38 | 51 | 28 | 0 | 0.00% | Undeletable | This study | ||
| IX (440 kb) | ScΔ(Chr 9-1) | 1–56 480 | Chr 9-1 | 56.4 | 35 | 223 | 20 | 10 | 50.00% | Deletable | This study |
| 87 850–102 249 | Chr 9-2 | 14.4 | 12 | 55 | 21 | 0 | 0.00% | Undeletable | This study | ||
| 175 096–187 852 | Chr 9-3 | 12.7 | 11 | 60 | 21 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 9-4) | 273 992–288 932 | Chr 9-4 | 14.9 | 11 | 66 | 24 | 12 | 50.00% | Deletable | This study | |
| ScΔ(Chr 9-5) | 321 054–343 678 | Chr 9-5 | 22.6 | 20 | 67 | 20 | 16 | 80.00% | Deletable | This study | |
| ScΔ(Chr 9-6) | 402 831–439 888 | Chr 9-6 | 37.0 | 29 | 250 | 20 | 12 | 60.00% | Deletable | This study | |
| X (745 kb) | ScΔ(Chr 10-1) | 1–49 918 | Chr 10-1 | 49.9 | 28 | 88 | 29 | 4 | 13.79% | Deletable | This study |
| 74 248–91 729 | Chr 10-2 | 17.5 | 15 | 79 | 22 | 0 | 0.00% | Undeletable | This study | ||
| 150 919–181 823 | Chr 10-3 | 30.9 | 18 | 53 | 25 | 0 | 0.00% | Undeletable | This study | ||
| 472 448–506 851 | Chr 10-4 | 34.4 | 19 | 54 | 25 | 0 | 0.00% | Undeletable | This study | ||
| 526 499–540 234 | Chr 10-5 | 13.7 | 13 | 66 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 607 564–633 651 | Chr 10-6 | 26.1 | 18 | 82 | 21 | 0 | 0.00% | Undeletable | This study | ||
| 655 969–692 506 | Chr 10-7 | 36.6 | 19 | 87 | 25 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 10-8) | 700 629–745 751 | Chr 10-8 | 45.1 | 25 | Deletable | Sugiyama et al. (14) | |||||
| XI (667 kb) | ScΔ(Chr 11-1) | 1–35 760 | Chr 11-1 | 35.7 | 18 | 59 | 20 | 4 | 20.00% | Deletable | This study |
| 188 434–204 755 | Chr 11-2 | 16.3 | 11 | 51 | 20 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 11-3) | 294 480–323 059 | Chr 11-3 | 28.5 | 22 | Deletable | Sugiyama et al. (14) | |||||
| ScΔ(Chr 11-4) | 457 598–478 769 | Chr 11-4 | 21.1 | 13 | 55 | 24 | 8 | 33.33% | Deletable | This study | |
| ScΔ(Chr 11-5) | 516 847–556 301 | Chr 11-5 | 39.4 | 24 | Deletable | Ueda et al. (17) | |||||
| ScΔ(Chr 11-6) | 606 478–666 816 | Chr 11-6 | 60.3 | 24 | Deletable | Ueda et al. (17) | |||||
| XII (1078 kb) | 1–36 402 | Chr 12-1 | 36.4 | 27 | 62 | 24 | 0 | 0.00% | Undeletable | This study | |
| 782 360–796 254 | Chr 12-2 | 13.9 | 14 | 77 | 25 | 0 | 0.00% | Undeletable | This study | ||
| 849 154–872 370 | Chr 12-3 | 23.2 | 20 | 93 | 24 | 0 | 0.00% | Undeletable | This study | ||
| 940 622–969 960 | Chr 12-4 | 29.3 | 21 | 71 | 25 | 0 | 0.00% | Undeletable | This study | ||
| 1 021 075–1 051 682 | Chr 12-5 | 30.6 | 13 | 75 | 21 | 0 | 0.00% | Undeletable | This study | ||
| 1 061 445–1 078 177 | Chr 12-6 | 16.7 | 17 | 48 | 21 | 0 | 0.00% | Undeletable | This study | ||
| XIII (924 kb) | 149 349–170 801 | Chr 13-1 | 21.5 | 16 | 92 | 20 | 0 | 0.00% | Undeletable | This study | |
| 300 686–323 520 | Chr 13-2 | 22.9 | 13 | 55 | 24 | 0 | 0.00% | Undeletable | This study | ||
| 460 669–490 680 | Chr 13-3 | 30.0 | 20 | 110 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 565 257–593 930 | Chr 13-4 | 28.7 | 20 | 52 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 603 532–655 150 | Chr 13-5 | 51.6 | 35 | 46 | 21 | 0 | 0.00% | Undeletable | This study | ||
| 754 644–785 961 | Chr 13-6 | 31.3 | 23 | 66 | 27 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 13-7) | 906 127–924 431 | Chr 13-7 | 18.3 | 17 | Deletable | Murakami et al. (23) | |||||
| XIV (784 kb) | 1–37 073 | Chr 14-1 | 37.0 | 26 | 44 | 22 | 0 | 0.00% | Undeletable | This study | |
| 62 859–82 820 | Chr 14-2 | 20.0 | 11 | 83 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 260 029–280 867 | Chr 14-3 | 20.8 | 13 | 52 | 23 | 0 | 0.00% | Undeletable | This study | ||
| 515 793–551 621 | Chr 14-4 | 35.9 | 28 | 55 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 590 250–614 516 | Chr 14-5 | 24.3 | 15 | 88 | 20 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 14-6) | 728 119–784 333 | Chr 14-6 | 56.2 | 28 | Deletable | Murakami et al. (23) | |||||
| XV (1091 kb) | ScΔ(Chr 15-1) | 1–41 505 | Chr 15-1 | 41.5 | 26 | Deletable | Sugiyama et al. (13) | ||||
| 98 213–117 310 | Chr 15-2 | 19.1 | 17 | 58 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 146 179–178 230 | Chr 15-3 | 32.1 | 14 | 81 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 207 919–249 764 | Chr 15-4 | 41.8 | 29 | 105 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 288 798–304 553 | Chr 15-5 | 15.8 | 13 | 57 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 337 904–367 536 | Chr 15-6 | 29.6 | 21 | 58 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 374 378–411 086 | Chr 15-7 | 36.7 | 26 | 87 | 20 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 15-8) | 476 130–500 179 | Chr 15-8 | 24.0 | 14 | Deletable | Sugiyama et al. (14) | |||||
| ScΔ(Chr 15-9) | 556 270–593 762 | Chr 15-9 | 37.5 | 20 | Deletable | Sugiyama et al. (14) | |||||
| 672 254–702 299 | Chr 15-10 | 30.0 | 16 | Undeletable | Sugiyama et al., (13) | ||||||
| 969 010–995 011 | Chr 15-11 | 26.0 | 18 | 65 | 23 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 15-12) | 1 043 649–1 091 291 | Chr 15-12 | 47.6 | 29 | Deletable | Sugiyama et al. (13) | |||||
| XVI (948 kb) | ScΔ(Chr 16-1) | 1–35 668 | Chr 16-1 | 35.6 | 22 | Deletable | Murakami et al. (23) | ||||
| 43 629–64 215 | Chr 16-2 | 20.6 | 19 | 50 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 332 333–366 459 | Chr 16-3 | 34.1 | 21 | 63 | 23 | 0 | 0.00% | Undeletable | This study | ||
| 434 789–466 542 | Chr 16-4 | 31.8 | 23 | 49 | 20 | 0 | 0.00% | Undeletable | This study | ||
| 547 237–574 201 | Chr 16-5 | 26.9 | 19 | 66 | 25 | 0 | 0.00% | Undeletable | This study | ||
| 675 669–699 987 | Chr 16-6 | 24.3 | 22 | 58 | 25 | 0 | 0.00% | Undeletable | This study | ||
| 756 832–792 577 | Chr 16-7 | 35.7 | 21 | 93 | 24 | 0 | 0.00% | Undeletable | This study | ||
| 824 875–860 801 | Chr 16-8 | 36.0 | 30 | 60 | 20 | 0 | 0.00% | Undeletable | This study | ||
| ScΔ(Chr 16-9) | 922 998–948 066 | Chr 16-9 | 25.1 | 18 | 162 | 25 | 21 | 84.00% | Deletable | This study |
aScΔ(Chr x-y): ScΔ represents S. cerevisiae deletion mutant, Chr x represents chromosome number and -y represents region number.
bx-y: x represents first nucleotide number of chromosomal region and -y represents last nucleotide number of chromosomal region.
cChr x-y: Chr x represents chromosome number and -y represent region.
dFrequency for obtaining segmental chromosomal deletion (number of segmental deletions/number of candidate transformants that were analyzed in karyotype.