Table 3. Characteristics of essential and dispensable chromosomal regions.
| Undeletable region by PCDa | Strainb | Undeletable regionc length (kb) | No. of genes (n) | First splitting transformants | Transformants with expected splitting/total no. of transformants examined for karyotype (%) | Second splitting transformants | Transformants with expected splitting/ total no. of transformants examined for karyotype (%) | Essential/ dispensable | References/ remarks |
|---|---|---|---|---|---|---|---|---|---|
| Chr 1-2 | 109.2–138.3 (29.1) | 19 | 164 | 5/5 (100) | 87 | 4/8 (50.0) | Essential | This study | |
| Chr 2-2 | 21.8–37.3 (15.5) | 14 | 83 | 3/4 (75.0) | 35 | 1/5 (20.0) | Essential | This study | |
| Chr 2-3 | 92.5–121.7 (29.2) | 18 | Essential | Harboring SL gene pair | |||||
| Chr 2-4 | 205.4–220.4 (15.0) | 9 | 190 | 4/4 (100) | 45 | 1/5 (20.0) | Essential | This study | |
| Chr 2-5 | 265.9–292.2 (26.3) | 15 | Essential | Harboring SL gene pair | |||||
| Chr 2-6 | 318.7–330.9 (12.2) | 9 | 47 | 13/15 (86.7) | 40 | 12/25 (48.0) | Essential | This study | |
| Chr 2-7 | 577.2–602.5 (25.3) | 19 | 76 | 8/10 (80.0) | 62 | 18/25 (72.0) | Essential | This study | |
| Chr 2-8 | 650.7–681.1 (30.4) | 22 | 111 | 3/5 (60.0) | 100 | 0.00% | ? | This study (not obtained) | |
| Chr 3-2 | ScΔ(Chr 3-2) | 68.9–83.6 (14.7) | 13 | 155 | 4/4 (100) | 86 | 1/4 (25.0) | Dispensable | This study |
| Chr 3-3 | 116.4–133.1 (16.7) | 12 | 35 | 4/4 (100) | 104 | 4/4 (100) | Essential | This study | |
| Chr 3-4 | 145.2–186.0 (40.8) | 31 | 65 | 4/4 (100) | 76 | 4/4 (100) | Essential | This study | |
| Chr 3-5 | 246.9–268.1 (21.2) | 17 | Essential | Harboring SL gene pair | |||||
| Chr 4-3 | 239.7–258.6 (18.9) | 13 | 91 | 4/4 (100) | 60 | 16/16 (100) | Essential | This study | |
| Chr 4-4 | ScΔ(Chr 4-4) | 309.9–334.2 (24.2) | 16 | 83 | 3/3 (100) | 65 | 2/4 (50.0) | Dispensable | This study |
| Chr 4-5 | 494.2–521.8 (27.6) | 24 | 150 | 4/4 (100) | 205 | 5/5 (100) | Essential | This study | |
| Chr 4-6 | 584.1–603.8 (19.7) | 14 | 43 | 3/3 (100) | 44 | 3/6 (50.0) | Essential | This study | |
| Chr 4-8 | 691.2–730.9 (39.7) | 20 | 112 | 5/5 (100) | 107 | 0.00% | ? | This study (not obtained) | |
| Chr 4-9 | 958.3–998.9 (40.6) | 31 | 146 | 3/3 (100) | 79 | 0.00% | ? | This study (not obtained) | |
| Chr 5-2 | 55.2–80.6 (25.4) | 22 | 167 | 6/6 (100) | 30 | 4/6 (67.0) | Essential | This study | |
| Chr 5-4 | 260.3–303.3 (43.0) | 27 | 65 | 3/3 (100) | 34 | 1/10 (10.0) | Essential | This study | |
| Chr 6-2 | 161.0–196.4 (35.4) | 23 | 58 | 5/6 (83.3) | 50 | 6/6 (100) | Essential | This study | |
| Chr 7-1 | 1–32.6 (32.6) | 20 | 47 | 4/8 (50.0) | 40 | 3/14 (21.0) | Essential | This study | |
| Chr 7-2 | 82.7–95.4 (12.7) | 11 | 108 | 4/4 (100) | 135 | 0.00% | ? | This study (not obtained) | |
| Chr 7-3 | ScΔ(Chr 7-3) | 129.7–171.4 (41.7) | 23 | 37 | 3/3 (100) | 115 | 5/5 (100) | Dispensable | This study |
| Chr 7-4 | ScΔ(Chr 7-4) | 190.8–210.0 (19.2) | 12 | 86 | 3/3 (100) | 82 | 4/6 (67.0) | Dispensable | This study |
| Chr 7-5 | 555.4–579.1 (23.6) | 19 | 143 | 3/3 (100) | 45 | 4/4 (100) | Essential | This study | |
| Chr 7-6 | 809.9–835.5 (25.6) | 21 | 121 | 3/3 (100) | 65 | 2/3 (66.7) | Essential | This study | |
| Chr 7-7 | 939.3–974.1 (34.8) | 21 | Essential | Harboring SL gene pair | |||||
| Chr 7-8 | 1055.7–1090.9 (35.2) | 22 | 205 | 5/5 (100) | 32 | 5/14 (35.7) | Essential | This study | |
| Chr 8-1 | 1–71.4 (71.4) | 47 | 210 | 3/4 (75.0) | 73 | 2/5 (40.0) | Essential | This study | |
| Chr 8-2 | 79.5–111.8 (32.3) | 28 | 66 | 3/3 (100) | 220 | 3/5 (60.0) | Essential | This study | |
| Chr 8-3 | 162.5–176.9 (14.4) | 11 | 97 | 3/4 (75.0) | 80 | 0.00% | ? | This study (not obtained) | |
| Chr 8-4 | 196.1–214.5 (18.3) | 17 | 274 | 5/5 (100) | 90 | 2/3 (66.7) | Essential | This study | |
| Chr 8-5 | 358.0–383.7 (25.7) | 28 | Essential | Harboring SL gene pair | |||||
| Chr 8-6 | 397.5–421.2 (23.7) | 13 | 54 | 5/5 (100) | 76 | 1/4 (25.0) | Essential | This study | |
| Chr 8-7 | 502.8–562.6 (59.8) | 38 | 83 | 5/5 (100) | 66 | 2/5 (40.0) | Essential | This study | |
| Chr 9-2 | 87.8–102.2 (14.4) | 12 | 60 | 4/4 (100) | 83 | 1/5 (20.0) | Essential | This study | |
| Chr 9-3 | 175.0–187.8 (12.7) | 11 | 123 | 4/4 (100) | 36 | 3/3 (100) | Essential | This study | |
| Chr 10-2 | 74.2–91.7 (17.5) | 15 | 245 | 5/5 (100) | 55 | 3/7 (42.8) | Essential | This study | |
| Chr 10-3 | 150.9–181.8 (30.9) | 18 | 183 | 4/4 (100) | 154 | 1/5 (20.0) | Essential | This study | |
| Chr 10-4 | ScΔ(Chr 10-4) | 472.4–506.8 (34.4) | 19 | 136 | 3/4 (75.0) | 33 | 1/5 (20.0) | Dispensable | This study |
| Chr 10-5 | 526.4–540.2 (13.7) | 13 | 104 | 4/4 (100) | 70 | 0.00% | ? | This study (not obtained) | |
| Chr 10-6 | 607.5–633.6 (26.1) | 18 | 49 | 5/5 (100) | 50 | 2/4 (50.0) | Essential | This study | |
| Chr 10-7 | 655.9–692.5 (36.6) | 19 | 88 | 3/3 (100) | 153 | 4/5 (80.0) | Essential | This study | |
| Chr 11-2 | 188.4–204.7 (16.3) | 11 | 57 | 4/4 (100) | 49 | 1/5 (20.0) | Essential | This study | |
| Chr 12-1 | 1–36.4 (36.4) | 27 | 145 | 4/6 (66.6) | 90 | 4/4 (100) | Essential | This study | |
| Chr 12-2 | ScΔ(Chr 12-2) | 782.3–796.2 (13.9) | 14 | 60 | 3/5 (60.0) | 87 | 3/5 (60.0) | Dispensable | This study |
| Chr 12-3 | 849.1–872.3 (23.2) | 20 | 44 | 4/5 (80.0) | 65 | 4/5 (80.0) | Essential | This study | |
| Chr 12-4 | 940.6–969.9 (29.3) | 21 | 172 | 3/4 (75.0) | 68 | 5/5 (100) | Essential | This study | |
| Chr 12-5 | 1021.0–1051.6 (30.6) | 13 | 123 | 3/3 (100) | 160 | 5/5 (100) | Essential | This study | |
| Chr 12-6 | 1061.4–1078.1 (16.7) | 17 | 249 | 6/10 (60.0) | 43 | 2/14 (14.2) | Essential | This study | |
| Chr 13-1 | ScΔ(Chr 13-1) | 149.3–170.8 (21.5) | 16 | 114 | 5/5 (100) | 55 | 6/9 (66.6) | Dispensable | This study |
| Chr 13-2 | 300.6–323.5 (22.9) | 13 | 182 | 5/5 (100) | 80 | 0.00% | ? | This study (not obtained) | |
| Chr 13-3 | 460.6–490.6 (30.0) | 20 | 96 | 6/6 (100) | 53 | 13/15 (86.7) | Essential | This study | |
| Chr 13-4 | 565.2–593.9 (28.7) | 20 | 83 | 5/5 (100) | 110 | 4/5 (80.0) | Essential | This study | |
| Chr 13-5 | 603.5–655.1 (51.6) | 35 | 118 | 3/3 (100) | 200 | 5/5 (100) | Essential | This study | |
| Chr 13-6 | 754.6–785.9 (31.3) | 23 | 78 | 5/7 (71.4) | 57 | 2/6 (33.3) | Essential | This study | |
| Chr 14-1 | 1–37.0 (37.0) | 26 | 66 | 1/9 (11.1) | 80 | 2/6 (33.3) | Essential | This study | |
| Chr 14-2 | 62.8–82.8 (20.0) | 11 | 120 | 5/5 (100) | 42 | 1/4 (25.0) | Essential | This study | |
| Chr 14-3 | 260.0–280.8 (20.8) | 13 | Essential | Harboring SL gene pair | |||||
| Chr 14-4 | 515.7–551.6 (35.9) | 28 | 135 | 3/3 (100) | 185 | 4/5 (80.0) | Essential | This study | |
| Chr 14-5 | 590.2–614.5 (24.3) | 15 | Essential | Harboring SL gene pair | |||||
| Chr 15-2 | 98.2–117.3 (19.1) | 17 | 37 | 2/5 (40.0) | 271 | 6/6 (100) | Essential | This study | |
| Chr 15-3 | 146.1–178.2 (32.1) | 14 | 59 | 5/5 (100) | 60 | 3/8 (37.5) | Essential | This study | |
| Chr 15-4 | 207.9–249.7 (41.8) | 29 | 86 | 3/3 (100) | 150 | 5/6 (83.3) | Essential | This study | |
| Chr 15-5 | 288.7–304.5 (15.8) | 13 | 160 | 5/5 (100) | 30 | 3/3 (100) | Essential | This study | |
| Chr 15-6 | 337.9–367.5 (29.6) | 21 | 297 | 4/4 (100) | 152 | 5/5 (100) | Essential | This study | |
| Chr 15-7 | 374.3–411.0 (36.7) | 26 | 76 | 7/7 (100) | 45 | 0.00% | ? | This study (not obtained) | |
| Chr 15-10 | 672.2–702.2 (30.0) | 16 | Essential | Sugiyama et al. (13) | |||||
| Chr 15-11 | ScΔ(Chr 15-11) | 969.0–995.0 (26.0) | 18 | 42 | 4/4 (100) | 63 | 5/5 (100) | Dispensable | This study |
| Chr 16-2 | 43.6–64.2 (20.6) | 19 | 265 | 4/4 (100) | 66 | 0.00% | ? | This study (not obtained) | |
| Chr 16-3 | 332.3–366.4 (34.1) | 21 | 164 | 3/3 (100) | 49 | 0.00% | ? | This study (not obtained) | |
| Chr 16-4 | 434.7–466.5 (31.8) | 23 | 66 | 5/5 (100) | 50 | 3/5 (60.0) | Essential | This study | |
| Chr 16-5 | ScΔ(Chr 16-5) | 547.2–574.2 (26.9) | 19 | 140 | 5/6 (83.3) | 95 | 5/5 (100) | Dispensable | This study |
| Chr 16-6 | 675.6–699.9 (24.3) | 22 | 95 | 5/5 (100) | 70 | 4/4 (100) | Essential | This study | |
| Chr 16-7 | ScΔ(Chr 16-7) | 756.8–792.5 (35.7) | 21 | 77 | 2/3 (66.7) | 80 | 6/6 (100) | Dispensable | This study |
| Chr 16-8 | ScΔ(Chr 16-8) | 824.8–860.8 (36.0) | 30 | 119 | 11/14 (78.6) | 50 | 5/6 (83.3) | Dispensable | This study |
aChr x-y: Chr x represents chromosome number and -y represents region.
bScΔ(Chr x-y): ScΔ represents S. cerevisiae deletion mutant, Chr x represents chromosome number and -y represents region number.
cx-y: x represents the left-hand side chromosomal region and -y represents the right-hand side of chromosomal region.