. 2014 Jul 31;42(15):9792–9806. doi: 10.1093/nar/gku660

Table 1. Sequence effect on the six-mismatch unpaired origins. Different end-structures were generated by hybridization of the corresponding oligonucleotides. The sequences of the first column correspond to the 5′ and 3′ six nucleotides at the termini of the displaced and template strands of the origins, respectively. The second column (Avg%) shows the utilization of the different origins as percentage respect to the 6m construct value to facilitate comparison. The percentage is the average of a minimum of three independent experiments. In the third column are listed the standard deviations corresponding to the averages in the second column.