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. 2014 Jul 23;42(15):10061–10072. doi: 10.1093/nar/gku663

Figure 2.

Figure 2.

Purification and verification of readthrough GST proteins. (A) The chromatograms show absorbance at 280 nm. The red curve corresponds to in-frame GST purification; the blue curve corresponds to GST-IXRI-TAG purification. The elution peaks appears in fraction 3. (B) SDS-PAGE gel stained by Coomassie Blue. Lane 1: whole-cell extract; lane 2: flowthrough after binding of the extract; lane 3: in-frame GST, and lane 4: purified readthrough protein, GST-IXRI-TAG. For lanes 3 and 4, 1.8 μg of protein was loaded on the gel. (C) The presence of the GST protein was checked by western blotting with an antibody against GST. Lane 1 corresponds to the whole-cell extract, lane 2 corresponds to the flowthrough after binding of the extract, lane 3 to in-frame GST, and lane 4 to purified readthrough protein, GST-IXRI-TAA. For lanes 3 and 4, 0.5 μg of protein was loaded on the gel.