Figure 5.

NOP58 and RBFOX2 snoRNA targets are form-dependent. The abundance of the different forms generated from each snoRNA was determined before and after the KD of either NOP58 or RBFOX2 and plotted relative to the number of nucleotides upstream of box C and downstream of box D. Shown are three examples representing snoRNAs producing only long (A), only short (B) or both long and short (C) forms. The snoRNA names are shown on top, while the enrichment of the short and long forms are illustrated at bottom. CPM and LF respectively indicate counts per million reads mapped and mock transfection (Lipofectamine). The data obtained after the transfection of two independent siRNAs targeting either NOP58 (blue bars) or RBFOX2 (red bars) and three mock transfections (black bars) are shown.