Figure 2.

Deletion of 22 amino acids from the interdomain linker does not hinder nucleic acid binding. (A) The 22 amino acids deleted (shaded gray) are structurally undefined in the Pot1pN+6mer and Pot1pC+6mer crystal structures and predicted to be unstructured (43). (B) Representative binding curves for wild-type and linker deletion proteins. Linker deletion has very little effect on Pot1-DBD binding to either 15mer (blue) or 12mer (green) ligands relative to wild-type (red). The slight increase in affinity is likely due to entropic effects.