Figure 1.

Interaction mapping of Rcl1p binding site on Bms1p. (A) Bms1p harbours from the N-terminus to the C-terminus a GTPase domain, a U3 snoRNA binding site (15), two coiled coil domains as defined by HHPRED server flanking a GAP domain and a C-terminal NLS. Several truncated versions of Bms1p have been produced in order to define the Rcl1p binding site on Bms1p. (B) Truncated derivatives of Bms1p tagged with 6 histidines at the N-terminus have been co-expressed with untagged Rcl1p. Cell lysates were incubated with cobalt-affinity resin and the bound fractions were analysed by SDS-PAGE and Coomassie-blue staining. (*) represents a known E. coli contaminating protein (Bifunctional polymyxin resistance protein Arn).